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. 2012 Oct 31;7(10):e48332. doi: 10.1371/journal.pone.0048332

Figure 4. Laminin, or β1 integrin activating antibody, HUTS-21, promote integrin signaling in RGCs in vitro.

Figure 4

P5 RGCs were cultured on laminin (a–d), or PDL (e–l) for 48 hours. HUTS-21 (i, k) or isotype control (j, l) antibodies (1 µg/ml) were added to RGCs cultured on PDL 12 hours after plating. Immunohistochemistry was performed with β1 integrin (a, e, i, j), FAK (b, f), [pY397]-FAK (c, g, k, l), or IgG control antibodies (d, h) and analyzed by confocal microscopy. 100–200 cells per condition were analyzed. A representative experiment is shown. A total of N = 5 experiments were performed. Scale bars: 10 µm.