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Probit curve used to calculate the limit of detection of the QIAsymphony RGQ method. Seven 0.5-log10 dilution series of CMV DNA positive whole blood in CMV DNA negative blood (lozenges) to yield concentrations ranging from 14 to 10000 CMV DNA copies/ml were tested 5 times each. The limit of detection at 95% was extrapolated from the sigmoid curve.
