Table 2. Mass determination of wild type and mutant FimP31–491 proteins.
Protein | Average mass (Da) | Difference (Da) | |
Calculated from sequence | Observed by MSa | calculated-observed | |
FimP31–491 | 53067 | 53033 | −34 |
FimP31–491 D230A | 53023 | 53006 | −17 |
FimP31–491 E452A | 53009 | 52993 | −16 |
MS, ESI-TOF mass spectrometry.