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. 2012 Oct 31;7(10):e48279. doi: 10.1371/journal.pone.0048279

Figure 5. Effect of MB and its derivatives in rotenone, IAA, and glucose oxidase toxicity assays.

Figure 5

(A) Effect of MB and derivatives against rotenone neurotoxicity in HT-22 cells. Cells were exposed to 5 µM rotenone for 24 hours in the presence of MB or its derivatives. Protective effect was observed in all compounds except chlorpromazine at the indicated concentrations (NR – Neutral Red; TB – Toluidine Blue O; MB – Methylene Blue; 2-C –2-chlorophenothiazine; Pheno – Phenothiazine; Chlor – Chlorpromazine Prom – Promethazine); # p<0.05 compared to media control. * p<0.05 compared to 5 µM rotenone in media (B) Effect of MB and derivatives against glucose oxidase neurotoxicity in HT-22 cells. Cells were exposed to 2 U glucose oxidase for 3 hours in the presence of MB or derivatives. No protective effect was observed in all compounds tested. Pyruvate (4 mM) was used as a positive control. # p<0.05 compared to media control. * p<0.05 compared to 5 U Glucose Oxidase in media (C) Effect of MB and derivatives against IAA neurotoxicity in HT-22 cells. Cells were exposed to 20 µM IAA for 24 hours in the presence of MB or derivatives. Protective effect was observed in all compounds at the indicated concentration. # p<0.05 compared to media control. * p<0.05 compared to 20 µM IAA in media.