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. 2012 Oct 31;7(10):e48279. doi: 10.1371/journal.pone.0048279

Figure 6. Effect of MB and derivatives on mitochondrial complexes activity.

Figure 6

(A) The effects of MB and its derivatives on the rate of cytochrome c reduction were recorded by measuring the change in absorbance of reduced cytochrome c with NADH as an electron donor (complex I-III activity) in isolated mitochondria. All compounds were tested at the concentration of 10 µM. MB and TB significantly increased complex I-III activity (the rate of cytochrome c reduction). Antimycin A (2 µg/ml), a complex III inhibitor, was used as a negative control. (B) Mitochondria complex II-III was measured by the change in absorbance of reduced cytochrome c with succinate as an electron donor in isolated mitochondria. All compounds were tested at the concentration of 10 µM. No effect was observed in all MB related compounds. Antimycin A (2 µg/ml) significantly decreased the rate of cytochrome c reduction. * p<0.05 compared to control.