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. 2012 Oct 31;7(10):e48379. doi: 10.1371/journal.pone.0048379

Figure 3. Effect of galectin-9 treatment on Con A-induced hepatitis.

Figure 3

Galectin-9 (100 µg per mouse) or PBS was administrated i.v. to Balb/c mice (n = 8 per group) 30 min before Con A injection (20 mg kg–1). (A) Serum ALT and AST levels were measured 24 h after Con A injection. (B) The livers were removed 24 h later. Paraffin sections were stained with hematoxylin and eosin (H&E). Representative liver sections were shown for each group, original magnificatios: ×200. (C) Liver DNA fragmentation was monitored by assessing histone ELISA, with or without pretreatment with galectin-9. (D) Time course of liver caspase 3–like activity are shown. The results (A, C, D) were presented as the mean ± SD of three separate experiments. **, p<0.01; *, p<0.05 vs PBS treatment; PBS, PBS treatment in Con A-treated mice; Gal-9, galectin-9 pretreatment in Con A-treated mice. Similar data presentation will appear in the subsequent figures.