Figure 7. Galectin-9 induced apoptosis of Con A–activated CD4⁺ T cells at day-5.

(A) The purity of isolated CD4⁺ T cells. (B) Spleen CD4⁺ T cells, isolated from untreated mice, were cultured with 10 µg/mL Con A for 5 days and then cultured for 24 hours at 37°C with or without 30 mM lactose followed by indicated doses of galectin-9, then apoptotic cells were assessed by FACS. (C) Time-course of galectin-9 (1 µM) induced apoptosis of day-5 Con A–activated T cells. (D) Absolute number of live cells after galectin-9 treatment, determined by Trypan blue dye exclusion. The results were presented as the mean ± SD of three separate experiments. *, P<0.05 vs PBS treatment.