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. 2012 Oct 31;7(10):e48461. doi: 10.1371/journal.pone.0048461

Figure 5. Guggulsterone-induced ERK1/2 phosphorylation and cell proliferation are mediated by Src, EGFR and post-EGFR signaling.

Figure 5

H508 cells (A and C) or SNU-C4 cells (B and D) were treated with 20 µM guggulsterone for 20 min at 37°C with or without pre-incubation with EGFR inhibitor AG1478 (AG) and PD168393 (PD16), MEK inhibitor PD98059 (PD98), Src kinase inhibitor PP2 and PI3 kinase inhibitor LY294002 (LY29). ERK1/2 phosphorylation was determined by immunoblotting with anti-phospho-ERK1/2 antibody. Immunoblotting for total ERK2 was used as a loading control. Immunoblots are representative of at least 3 separate experiments. H508 cells (E) or SNU-C4 cells (F) were incubated for 5 days at 37°C with guggulsterone (10 µM) in the presence or absence of Src inhibitor PP2, EGFR inhibitors PD16 and AG, MEK inhibitor PD98 and PI3 kinase inhibitor LY29. Cell proliferation was measured as described in Materials and Methods. Values are means ± SE from at least 3 separate experiments *p<0.05 vs. guggulsterone alone (Student’s t-test).