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. 2012 Oct 31;7(10):e48366. doi: 10.1371/journal.pone.0048366

Figure 1. The method used to obtain reproducible data for microarray analysis conducted on serum-extracted samples.

Figure 1

A. NL patients’ serum were sampled twice. In the first, RNA was extracted first from untreated serum, and then extracted again from serum treated with exoquick. In the second serum sample, RNA was also extracted from both untreated serum and serum treated with exoquick. Microarray analysis was conducted for RNA in a total of four samples. B. Reproducibility test of microarray data. Scatter plots comparing non- normalized signal intensities of miRNAs in two independent experiments from human total serum and exosome rich fraction. Red and black denotes high and low miRNA expressions respectively. Total serum extracted first, versus exosome rich fraction first (left), total serum extracted first versus second (middle), and exosome rich fraction extracted first versus second (right). C. Pearson’s pairwise correlations of signal intensities of miRNAs from human total serum and exosome rich fraction. D. Western blot was performed for untreated serum, serum extracted by exoquick and exosome fraction from PNT-2, using anti-CD63.