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. 2012 Oct 31;7(10):e48384. doi: 10.1371/journal.pone.0048384

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© 2012 Lv et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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BALB/c mice were divided into 2 groups, the control group (C) and the abortion medicine-treated group (T). Mice in the C group were subjected to 3 normal pregnancies, termed C_G1P1 (gravida 1 and parity 1), C_G2P2 (gravida 2 and parity 2), and C_G3P3 (gravida 3 and parity 3). Mice in the T group were treated with RU 486 on day 8.5 of pregnancy. On E13.5, C_G1P1_E13.5, C_G3P3_ T_G2P1, and T_G3P1 mice were sacrificed to compare their mid-pregnancy status, the frequency of fetal resorption, and the expression of placental function-related genes. The expression of metabolism-related genes in the F1 generation (C_G1P1 mated with T_G3P1) was also compared.