Figure 2. Mutation of cysteine 111 to serine or tyrosine differentially affects inclusion formation.

CHO cells were transiently transfected with expression plasmids for each mutant and imaged 24 or 48 hours later as described in Figure 1. Mutation of cysteine 111 to serine does not induce inclusion formation whereas the disease associated mutation of cysteine to tyrosine produces inclusion-like structures at a high frequency. For comparison, cells expressing WT SOD1:YFP fusion proteins are also shown.