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. 2012 Oct 31;7(10):e47838. doi: 10.1371/journal.pone.0047838

Figure 3. Simultaneous mutation of cysteines 6 and 111.

Figure 3

CHO cells were transiently transfected with expression plasmids for each mutant and imaged 24 or 48 hours later as described in Figure 1. For the C6G-C111S and C6G-C111Y mutants (Panels A–D), the rate of inclusion formation was slower than that of the positive control mutant A4V (Panels E and F). At 48 hours, the frequency of inclusion-like structures in cells expressing the double mutant C6G-C111S remained low compared to C6G-C111Y (see Table 1).