Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Oct 31;7(10):e46783. doi: 10.1371/journal.pone.0046783

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 Lin et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

A. Mucoviscosity of K. pneumoniae NTUH-K2044 wild type, ΔmagA mutant, magA (G308A, G310A, G334A, G337A, R290A, P305A, H323A and N324A) site-directed mutant strains and complementation strains. The mucoviscosity determined by centrifugation was represented by OD₆₀₀ of three independent experiments (mean±SD). (wild type vs. ΔmagA, P<0.0001; wild type vs. magA-G308A, P = 0.0012; wild type vs. magA-G334A, P = 0.0097; wild type vs. magA-R290A, P = 0.0012; wild type vs. magA-H323A, P = 0.0012; ΔmagA vs. ΔmagA complementation, P = 0.0070; magA-G308A vs. magA-G308A complementation, P = 0.0034; magA-G334A vs. magA-G334A complementation, P = 0.0181; magA-R290A vs. magA-R290A complementation, P = 0.0029; magA-H323A vs. magA-H323A complementation, P = 0.0031; Student's t test) **P<0.01 *P<0.05. B. CPS production of K. pneumoniae NTUH-K2044 wild type, ΔmagA mutant, magA (G308A, G310A, G334A, G337A, R290A, P305A, H323A and N324A) site-directed mutant strains and complementation strains. The amount of CPS was represented by OD₅₂₀ of three independent experiments (mean±SD). (wild type vs. ΔmagA, P = 0.02; wild type vs. magA-G308A, P = 0.0098; wild type vs. magA-G334A, P = 0.0048; wild type vs. magA-R290A, P = 0.0109; wild type vs. magA-H323A, P = 0.0173; ΔmagA vs. ΔmagA complementation, P = 0.0392; magA-G308A vs. magA-G308A complementation, P = 0.0332; magA-G334A vs. magA-G334A complementation, P = 0.0040; magA-R290A vs. magA-R290A complementation, P = 0.0349; magA-H323A vs. magA-H323A complementation, P = 0.0239; Student's t test) **P<0.01 *P<0.05. C. Double immunodiffusion assays of rabbit anti-K. pneumoniae K1 serum with capsular extracts of different K. pneumoniae strains. The rabbit anti-K1 serum from Statens Serum Institute is in the center well and capsular extracts of overnight-cultured K. pneumoniae strains are in peripheral wells. The names of the strains are labeled in the peripheral wells. A red precipitation line near the peripheral well is considered as a positive reaction.