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. 2012 Oct 31;7(10):e46783. doi: 10.1371/journal.pone.0046783

Figure 3. Phagocytosis resistance of magA point mutants.

Figure 3

Phagocytosis resistance of K. pneumoniae NTUH-K2044 wild type, ΔmagA mutant, magA (G308A, G310A, G334A, G337A, R290A, P305A, H323A and N324A) site-directed mutant strains. K. pneumoniae was plated on an SM agar plate, and 5,000 Dictyostelium cells in 2 µl of HL5 media were added on the plate center for four-day incubation at 22°C. The formation of phagocytic plaques are observed in ΔmagA, G308A, R290A and H323A mutants.