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. 2012 Oct 31;7(10):e47621. doi: 10.1371/journal.pone.0047621

Figure 2. Expression and localization of cortical marker proteins in wild-type and mutant strains.

Figure 2

In each panel, a montage of representative cells is shown. (A) Localization of the bipolar marker proteins Bud8, Bud9, and Rax2. Wild-type diploid strain YEF473, bud13Δ/bud13Δ strain STY254, and ist3Δ/ist3Δ strain STY260 were transformed with plasmid YEpGFP*-BUD8F, YEpGFP*-BUD9, or RAX2-GFP, and cells grown to exponential-phase in SC-Leu or SC-Ura medium were examined for the localization and intensity of GFP fluorescence. (B) Expression and localization of the haploid-specific axial-marker protein Axl1. Wild-type MAT a strain STY604, wild-type MAT a /MATα strain STY612, and MAT a /MATα strains homozygous for bud13Δ (STY611), ist3Δ (STY610), or pml1Δ (STY619), each of which expresses AXL1-GFP from the chromosomal AXL1 locus, were grown to exponential phase in SC medium and examined for the expression and localization of GFP fluorescence. Exposure time and scaling factor were identical for each image in panel B.