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. 2012 Oct 31;7(10):e48115. doi: 10.1371/journal.pone.0048115

Figure 7. The role of PI3K/Akt signaling in the protection by fasudil was evaluated by using an Akt inhibitor, LY294002.

Figure 7

Sham group, I/R group and I/R+fasudil group were either given LY294002 or placebo (n = 6 for each group rats treated with LY294002 for both time points, all the other rats are the same as shown in above). Western blotting was performed, targeting phosphorylation levels of Akt (representative protein bands shown in Panel A and quantified in bar graph in Panel B); JAK2 (Panel C & D), STAT3 (Panel E & F) and PLB (Panel G & H). SERCA protein expression levels (Panel I & J) were also analyzed. All data expressed as mean±SD. * denotes P<0.05 vs. Sham groups; †, P<0.05 vs. I/R group at the same time point and ‡, P<0.05 vs. I/R+fasudil group at the same time point.