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. 2012 Oct 31;7(10):e46889. doi: 10.1371/journal.pone.0046889

Figure 4. LSG-induced SEPT9 expression inhibits RhoA activity and peripheral actin distribution.

Figure 4

(A) Gene expression profiles for SEPT7, SEPT9, and SEPT11 in ECs cultured on LSG and HSG for 24 h. Data represent mean ± SEM (Student's t-test; * p<0.05, ** p<0.01). (B) Gene expression profile of SEPT9 after transfection with sh-SEPT9 (left panel). RhoA activity level after transfection with sh-SEPT9 was followed by RBD-pull down assay (right panel). * p<0.05 in comparison with the corresponding controls on HSG and LSG. # p<0.05 for comparison between control and sh-SEPT9 on LSG. (n = 3). (C) Phase microscopy images of ECs seeded on HSG or LSG for 24 h in response to sh-SEPT9 transfection. Scale bar = 50 µm. (D) ECs with control and sh-SEPT9 transfection were seeded on LSG for 3 h. Actin was stained with FITC-phalloidin and the cellular distribution was quantified by image analysis using the intensity ratio profile program (Fig. S1). * p<0.05 for comparison between control and sh-SEPT9 at successive normalized radial positions from the cell center (0) to the periphery (1). n = 17 cells. Scale bar = 50 µm.