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. 2012 Oct 31;7(10):e48075. doi: 10.1371/journal.pone.0048075

Figure 3. EF24 inhibits angiogenesis and tumor cell survival signaling in liver cancer.

Figure 3

(a). Lysates from EF24-treated (4 µM,) cells (Hepa1-6 and H22) used for western blot analysis on the expressions of ERK, p-ERK, Akt, p-Akt and COX-2. (b). HUVEC cells was treated with the indicated concentrations of EF24 for 24 h and 48 h. Cell growth was determined by cell counting kit-8 assay. (c). The proliferation of cells was also measured by crystal violet assay. (d). Tumor tissue lysates was used for western blotting to detect the levels of p-ERK, COX-2 and VEGF.