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. 2012 Oct 31;7(10):e48075. doi: 10.1371/journal.pone.0048075

Figure 4. EF24 inhibits liver cancer growth and induces apoptosis both in subcutaneous HCC tumor model and orthotopic HCC models.

Figure 4

(a). EF24 inhibits mice subcutaneous tumor growth in vivo. (b). Tumor size was measured after treated for three weeks. (c). EF24-treated animals resulted in a significantly lower tumor weight when compared with controls. (d). Tumor tissue removed from the animals were used for western blot analysis on the expressions of caspase-3, Cyclin B1, Cdc2, Bcl-2 and Bax. (e). Liver removed from the orthotopic HCC models, treated or untreated with EF24 for three weeks. (f). EF24 treatment groups significantly reduced the liver/body weight ratio compared to the control. (g). After treatmeant for three weeks, tumor tissue sections were stained with haematoxylin and eosion (HE). (h). Relative areas occupied by tumors in livers were calculated. (i). Tumor sections were stained with an anti-Ki-67 Ab to detect proliferating cells. (j). Cells expressing Ki-67 were counted to calculate the proliferation index, Assay was done in triplicate and p<0.01 is denoted by “*”.