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. 2012 Oct 31;7(10):e47569. doi: 10.1371/journal.pone.0047569

Figure 2. Cells in sternotomy wound-fluid are predominantly PMN.

Figure 2

Wound cells isolated from WF were subjected to flow cytometry analysis. A, forward and sideward scatter (FS/SS) and flow cytometry analysis to sub-fraction wound and blood leukocytes. Based on FS/SS characteristics of blood leukocytes (PB, before surgery), three major cell populations were gated: gate i, granulocytes; gate ii, monocytes; gate iii, lymphocytes; B, quantification of cells in each of the three gates (i–iii). Solid bar, blood at baseline; open bars, WF cells at 48 h post-surgery. Data are mean ±SD, n = 4. C, Neutrophil count expressed in % of total leukocytes as determined by microscopic differential count. Data presented as mean± SD, n = 7. *p<0.05 compared to PB.