Figure 2. ScARPs are surface exposed.

(A) Western immunoblot analysis of ScARPs from S. citri GII3. Spiroplasmas were incubated either with the reaction buffer alone (−) or with 1 mg/ml trypsin (+), for 1, 4, and 24 h. Proteins were probed with MAb 10G3. (B–D) Western immunoblot analysis of S. citri GII3 treated by proteinase K during 10 min at various concentrations. Proteins were probed with anti-P32 PAbs (B), MAb 10G3 (C), and anti-Rep3d PAbs (D).