Figure 1. Rad52 is phosphorylated in S phase cells exposed to UVA radiations.
(A) Protein extracts analyzed by immunoblot with anti-GFP antibodies (upper panel) to detect Rad52 protein from rad52YFP cells synchronized in early S phase by HU (block), collected in water and released in fresh medium after 0 or 400 kJ/m2 UVA for different time points. “asyn” indicates cells in log phase. Star refers to the same membrane colored with Rouge Ponceau to serve as loading control. An aliquot of cells at different time points was used to estimate cell cycle progression by FACS analysis and by scoring the percentage of cells that passed mitosis (cells with 2 nuclei). 1C and 2C indicate DNA content. (B) The change in mobility shift of Rad52 protein upon UVA is abolished by treatment with l phosphatase (PP).