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Osteocalcin immunostaining (A), immunoblotting (B) and densitometric results (C) in porcine aortic valve leaflets subjected to TAV and BAV WSS (F: fibrosa; green: positively stained cells; blue: cell nuclei; *: p<0.05 vs. fresh control; #: p<0.05 vs. TAV WSS; +: p<0.05 vs. NC-BAV WSS).
