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. 2012 Oct 31;7(10):e48745. doi: 10.1371/journal.pone.0048745

Figure 1. Transcript- and genomic level validation of the fusion genes.

Figure 1

A. Validation of the fusion genes from BT-474 and MCF-7 on the cDNA level by RT-PCR. B. Genomic DNA sequence at the fusion gene break point of THRA-AC090627.1 (top), TOB1-SYNRG (middle) and MED1-ACSF2 (bottom). Chromosomal positions of the fusion break points are indicated by black arrows. Gene and transcript structures as well as nucleotide sequences at the break points are drawn in blue for 5′ and in red for 3′ partner genes. Gene structures above and below chromosome coordinates imply forward and reverse strand, respectively. Transcript structures of gene fusions are indicated below the gene structures and connected with gray lines. Genomic DNA sequence at the break point (indicated by asterisk) is shown below the transcript structures. Black color indicates nucleotides that match to both (THRA-AC090627.1, MED1-ACSF2) or neither partner genes (TOB1-SYNRG).