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. 2012 Oct 31;7(10):e48654. doi: 10.1371/journal.pone.0048654

Figure 5. Invasiveness of neuroblastoma cells after contact with human mesenchymal stem cells.

Figure 5

SH-SY5Y, Htla-230 and GI-LI-N NB cells incubated or not with hMSCs in a transwell system for 24 hours, were placed in the upper wells on Matrigel®-coated invasion chambers and separated by an 8 μm membrane from CXCL12 or bone marrow sample. After 24 hours, NB cells migrated into the lower chamber were stained and counted after May Grunwald-Giemsa. Panels A–C show the invasiveness of SH-SY5Y (Panel A), GI-LI-N (Panel B) and Htla-230 (Panel C) to CXCL12. Panels D shows the invasiveness of SH-SY5Y to CXCL12 in presence or absence of CXCR4 antagonist AMD3100 that was added to some wells. Panel E shows the invasiveness of SH-SY5Y to the bone marrow of an healthy donor. The data are expressed as mean values obtained from the count of 10 fields/well. Bars are SD. Statistical analysis was performed by Unpaired t test with Welch's correction.