Fig. 4.
DHA-Bodipy has higher fluorescence lifetimes in ordered domains compared to disordered domains. Fluorescence lifetime of the DHA-Bodipy probe was measured using time-resolved fluorescence anisotropy in lipid vesicles of defined composition at (A) 37°C, (B) 23°C and (C) 10°C. POPC/Chol, POPC and SDPC, respectively, represent highly ordered, disordered and highly disordered membrane domains. Data are average±S.E. from three independent experiments. Asterisks indicate significance from SDPC: **P<.01; ***P<.001.