Figure 3.

Characteristics of Retinal Clone Size Evolving over Time

(A) Size distributions of the clones induced at 24 hpf and recorded at 48 hpf (left) or at 72 hpf (right), highlighting numbers of even (green) versus odd (blue) clones.

(B) Size distributions of clones photoconverted at various times. The mean and SD are indicated (n = 64, 169, and 163 for 24, 32, and 48 hpf; NS, not significant; ^∗p < 0.05, Student’s t test). Clones photoconverted at 48 hpf display significantly fewer three-cell than four-cell clones (two-proportion z test, p = 0.011).

(C) After photoconversion at 32 hpf, the size of the resulting subclone at 72 hpf (y axis, depicted as the magenta cells in the schematic shown in inset) is, on average, approximately inversely correlated with the total size of the parent clone at 32 hpf (x axis, shown as the enclosed green and magenta cells in inset). The points show measurements from individual clones, while the mean and SEM are shown in purple. If the fate of RPCs is independent of clonally related cells, the average size of the subclone after photoconversion is predicted to vary as N/n where N denotes the average total clone size at 72 hpf and n denotes the average total clone size at 32 hpf. Indeed, the measured averages (purple) are broadly consistent with this prediction (orange line).