Figure 2.

Immunoblot analysis of total soluble proteins extracted from greenhouse-grown control maize and chamber-grown W22 and bsd2-m1 leaves harvested after approximately 8 h of darkness (D), after 2 h of low-light (LL) illumination (approximately 100–130 μmol m⁻² s⁻¹), and after a further 2 h of illumination at a more moderate light (ML) intensity (approximately 400–450 μmol m⁻² s⁻¹). It should be noted that the trace amounts of Rubisco large subunit depicted for bsd2-m1 in this figure were observed infrequently and were not functional in in vitro, ¹⁴C-based Rubisco assays (see Table II and Discussion). M_cyt, Mesophyll-cell specific, located in the cytosol; M_chl, mesophyll-cell specific, located in the chloroplast; BS_chl, bundle-sheath-cell specific, located in the chloroplast; LSU, large subunit.