Figure 1.

Seqlogo describing the SigB binding site model derived in this study. This model is virtually identical to the Bc ATCC14579 SigB binding site derived by van Schaik et al.[44], except that the A at position 8 has a stronger signal, and the spacer region between the −35 and −10 sequences is shorter by 1bp. Since the Bc ATCC14579 binding site was derived from 10 sequences, while our SigB binding site model relied on 130 sequences, our model may be somewhat more robust, although the differences appear negligible. A model of the binding site for the orthologous B. subtilis SigB protein also is very similar, lacking only the strong A at position 7; the B. subtilis SigB binding site was extracted from a training set of 63 sequences [47].