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Total ion chromatograms (TICs) of unsulfated saturated chondroitin oligosaccharides up to 16-mers in negative ion mode separated by reversed-phase ion-pairing (RPIP)-HPLC. According to Thanawiroon and Linhardt,17 the presence of tributylamine may result in peak doubling, presumably through the resolution of the α and β anomeric forms of the individual oligosaccharides.
