Table 1.
Calculated monoisotopic m/z values for anions derived from fully saturated unsulfated chondroitin oligomer species separated by means of reversed-phase ion pairing (RPIP)-HPLC, and calculated MS/MS C1 product ions derived from precursor ions by glycosidic cleavage (according to the nomenclature of Domon and Costello14). The main ions detected for each oligosaccharide are highlighted in bold. m.m.=molecular mass. RT=retention time
| Possible ions (charge) m/z |
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chondroitin oligomers | m.m. | (−1) | (−2) | (−3) | (−4) | (−5) | (−6) | (−7) | (−8) |
| 2-mers (RT=6.3 min) | |||||||||
| GlcA-GalNAc | 397.1 | 396.1 | |||||||
| C1 product | 194.0 | 193.0 | |||||||
| 4-mers (RT=7.8 min) | |||||||||
| [GlcA-GalNAc]2 | 776.2 | 775.2 | 387.1 | ||||||
| C3 product | 573.1 | 572.1 | 285.1 | ||||||
| 6-mers (RT=10.2 min) | |||||||||
| [GlcA-GalNAc]3 | 1155.3 | 1154.3 | 576.7 | 384.1 | |||||
| C5 product | 952.2 | 951.2 | 474.6 | 316.1 | |||||
| 8-mers (RT=13.6 min) | |||||||||
| [GlcA-GalNAc]4 | 1534.4 | 1533.4 | 766.2 | 510.5 | 382.6 | ||||
| C7 product | 1331.3 | 1330.3 | 664.2 | 442.4 | 442.4 | ||||
| 10-mers (RT=18.6 min) | |||||||||
| [GlcA-GalNAc]5 | 1913.5 | 1912.5 | 955.8 | 636.8 | 477.4 | 381.7 | |||
| C9 product | 1710.4 | 1709.4 | 853.7 | 568.8 | 426.4 | 340.9 | |||
| 12-mers (RT=23.2 min) | |||||||||
| [GlcA-GalNAc]6 | 2292.6 | 2291.6 | 1145.3 | 763.2 | 572.2 | 457.5 | 381.1 | ||
| C11 product | 2089.5 | 2088.5 | 1043.3 | 695.2 | 521.1 | 416.7 | 347.1 | ||
| 14-mers (RT=26.5 min) | |||||||||
| [GlcA-GalNAc]7 | 2671.7 | 2670.7 | 1334.9 | 889.6 | 666.9 | 533.3 | 444.3 | 380.7 | |
| C13 product | 2468.6 | 2467.6 | 1232.8 | 821.5 | 615.9 | 492.5 | 410.3 | 351.5 | |
| 16-mers (RT=29.2 min) | |||||||||
| [GlcA-GalNAc]8 | 3050.8 | 3049.8 | 1524.4 | 1015.9 | 761.7 | 609.2 | 507.5 | 434.8 | 380.4 |
| C15 product | 2847.7 | 2846.7 | 1422.4 | 947.9 | 710.7 | 568.3 | 473.5 | 405.7 | 354.8 |