TABLE 1.
Lens protein bound fluorescence and advanced glycation end products (AGEs) in transgenic-mouse model and wild-type mouse at age 6, 9, and 12 months
| Wild-type mouse lens |
Transgenic-mouse lens |
Human lens |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Fluorescence and AGEs |
6 mos | 9 mos | 12 mos |
P value with age |
6 mos | 9 mos | 12 mos |
P value with age |
Reported Human lens results with age |
Ref. |
| λ335/385(unit/unit protein) | 6.57 ± 2.02 | 9.11 ± 0.97 | 13.91 ± 1.07 | 0.05 | 15.04 ± 2.53 | 18.97 ± 3.36 | 45.50 ± 11.17 | 0.0001 | Significant | 35 |
| λ370/440(unit/unit protein) | 5.20 ± 1.76 | 5.88 ± 1.02 | 6.95 ± 1.53 | N.S | 8.16 ± 3.41 | 10.89 ± 3.37 | 17.59 ± 4.20 | 0.0001 | Significant | 36 |
| K2P(pmol/µmol leu equi) | 0.00 | 0.10 ± 0.00 | 0.11 ± 0.0 | N.S | 3.08 ± 1.33 | 3.50 ± 1.51 | 19.94 ± 4.27 | 0.0001 | Significant | 18 |
| CML(µmol/mol lysine) | 159.73 ± 13.05 | 228.82 ± 30.47 | 253.03 ± 35.81 | 0.01 | 367.35 ± 76.96 | 444.41 ± 101.14 | 583.33 ± 105.37 | 0.005 | Significant | 14 |
| CEL(µmol/mol lysine) | 205.37 ± 6.71 | 48.10 ± 5.11 | 53.30 ± 9.75 | N.S | 86.58 ± 14.32 | 65.04 ± 18.80 | 77.62 ± 28.72 | N.S | Significant | 37 |
| Furosine(µmol/mol lysine) | 1035.95 ± 175.88 | 1383.21 ± 267.32 | 1250.16 ± 263.30 | N.S | 1323.13 ± 319.68 | 1391.42 ± 272.52 | 1138.92 ± 215.27 | N.S | N.S | 14 |
| Vesperlysine A (pmol/µmol leu equi) | 0 | 0 | 0 | N.S | 2.93 ± 1.35 | 10.35 ± 1.59 | 14.17 ± 2.29 | 0.008 | Significant | 14 |
| Pentosidine (pmol/µmol leu equi) | 0.0006 ± 0.0 | 0.056 ± 0.053 | 0.111 ± 0.071 | N.S | 0.44 ± 0.27 | 1.13 ± 0.48 | 1.94 ± 0.79 | 0.0001 | Significant | 9 |
| Lens discoloration | no | no | no | no | mild | yellow | Yellow around 55 yrs | 20 | ||
Abbreviations: CEL, carboxyethyllysine; CML, carboxymethyllysine; K2P, ;N.S., not significant.