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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Biochem Biophys Res Commun. 2012 Sep 16;427(1):178–184. doi: 10.1016/j.bbrc.2012.09.038

Figure 3.

Figure 3

Bone marrow-derived macrophages were pre-incubated for 15 min with the calmodulin inhibitor W7 (25 μM), treated with TNFα (TNF, 10 ng/ml) for the indicated times and then processed for immunodetection of phospho-AKT (Ser473; 60 kDa, A), phospho-IκBα (Ser32/36, 40 kDa, B) or phospho-p38MAPK (pP38, Thr180/Tyr182Ser473; 43 kDa, C) in whole cell lysates. Membranes were reprobed for total AKT, GAPDH or total p38MAPK to control for protein loading. Blots are representative from three independent experiments.