Table 1.
Expression of the surface markers on mouse BMDCs: untreated cells, cells pulsed with LPS (positive control) or with different Trichinella spiralis antigens for 48 h. Results were obtained by flow cytometry and represent a mean value ± SEM of the results from five independent experiments, performed in triplicates. Mean fluorescent intensity (MFI) and per cent of cells positive for the different surface markers (% pos). *P < 0.05, **P < 0.01, ***P < 0.001 represent statistically significant difference to the nonstimulated BMDCs (cultured in medium)
MHC II | ICAM 1 | CD80 | CD86 | OX40L | ||||||
---|---|---|---|---|---|---|---|---|---|---|
% pos (SEM) | MFI (SEM) | % pos (SEM) | MFI (SEM) | % pos (SEM) | MFI (SEM) | % pos (SEM) | MFI (SEM) | % pos (SEM) | MFI (SEM) | |
Non-pulsed DCs (Medium) | 27 (2) | 2387 (108) | 245 (2) | 14 (1) | 34 (2) | 801 (38) | 31 (1) | 667 (36) | 22 (2) | 136 (7) |
DCs pulsed with LPS | 60 (3)*** | 4068 (128)*** | 35 (7)* | 19 (2)* | 61 (6)*** | 1621 (42)*** | 60 (4)*** | 1294 (75)*** | 35 (7)* | 176 (23)* |
DCs pulsed with MLCr | 34 (4)* | 2885 (280)* | 27 (3) | 16 (2) | 40 (4)* | 969 (104)* | 37 (3)* | 779 (52)* | 26 (2)* | 164 (17)* |
DCs pulsed with HMC-Ag | 57 (7)*** | 4237 (275)*** | 33 (4)* | 18 (3)* | 57 (6)*** | 2667 (265)*** | 60 (4)*** | 1932 (165)*** | 30 (4)* | 180 (23)* |
DCs pulsed with ES LI | 44 (4)** | 3221 (211)** | 25 (4) | 17 (2) | 55 (3)*** | 1592 (84)*** | 54 (3)*** | 1095 (109)*** | 24 (2) | 157 (27) |
DCs pulsed with Ad ES | 33 (4)* | 3027 (332)* | 28 (2) | 15 (2) | 48 (3)*** | 1710 (101)*** | 60 (5)*** | 1146 (122)*** | 25 (2) | 143 (18) |
DCs pulsed with NBL | 32 (3)* | 2939 (254)* | 28 (3) | 16 (2) | 58 (4)*** | 1608 (70)*** | 49 (5)** | 1078 (128)** | 25 (2) | 141 (27) |