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Multiple alignment of PR amino acid sequences of eBAC31A08 and representative hot spring sequences from each hp clade. Positions are based on the eBAC31A08 protein numbering system (3). Residues 97 and 108 are marked with an arrow, and position 105 is boxed. Transmembrane helices (3) are indicated, as well as the position of the degenerate primers (gray boxes) and the specific primers designed in this study (underlined sequences).
