Fig 1.

In situ visualization of Sodalis clusters present in the tsetse gut. (A) Cross sections of tsetse gut tissue stained with DAPI. DNA from host epithelial tissue and Sodalis cells all stains blue. Bar, 50 μm. (B) Visualization of Sodalis clusters in the lumen of the tsetse gut using fluorescent in situ hybridization. Tissues were probed with a rhodamine-labeled oligonucleotide specific for Sodalis 16S rRNA (red). Bar, 50 μm. Autofluorescent blood meal-derived erythrocytes surround Sodalis cells. Bars, 50 μm. (C) Panels A and B merged to better orient the position of Sodalis cell clusters in relation to tsetse gut epithelial tissues. In each of panels A, B, and C, the most prominent cluster of Sodalis cells is indicated by a white arrow. (D to F) Enlarged views of the Sodalis clusters shown in panels A to C, respectively. Bars, 10 μm.