Fig 3.

Generation of a Sodalis ompA mutant strain. (A) PCR amplification of the ompA gene in the Sodalis parent strain (Sodalis PT; lane 2) and Sodalis ompA mutants (Sodalis ΔOmpA; lane 3). The ∼1-kb band shift is the result of intron insertion into the ompA locus of the Sodalis chromosome. (B) A Western blot showing OmpA expression in Sodalis PT cells (lane 1) but not in their Sodalis ΔOmpA derivatives (lane 2). (C and D) Sodalis PT and Sodalis ΔOmpA growth curves in MM medium and blood, respectively. Sodalis ΔOmpA does not exhibit a growth defect when cultured in either medium.