. 2012 Nov;78(21):7706–7719. doi: 10.1128/AEM.02157-12

Table 4.

Differentially expressed stress resistance genes in E. coli O157:H7 curli variants

Group and gene name	ID	Product	COG^a	Fold upregulation in RM6607W^b
Acid resistance genes
gadC	Z2216	Acid sensitivity protein	COG0531E	99.2
yhiM	Z4890	Hypothetical protein related to acid resistance	NA	101.1
yhiD	Z4920	Putative Mg²⁺ transport ATPase	COG1285S	5.3
hdeB	Z4921	Acid resistance protein	NA	100.8
hdeA	Z4922	Acid resistance protein	NA	83.2
hdeD	Z4923	Acid resistance membrane protein	COG3247S	70.6
gadA	Z4930	Glutamate decarboxylase isozyme	COG0076E	100.6
Heat shock resistance genes
dnaK	Z0014	Chaperone Hsp70, autoregulated heat shock protein	COG0443O	3.0
dnaJ	Z0015	Chaperone with DnaK, heat shock protein	COG0484O	3.0
htpG	Z0590	Chaperone Hsp90, heat shock protein C 62.5	COG0326O	2.7
cbpA	Z1418	Curved DNA-binding protein, functions closely related to DnaJ	COG0484O	2.8
grpE	Z3907	Heat shock protein GrpE	COG0576O	2.0
groES	Z5747	Cochaperonin GroES	COG0234O	3.7
groEL	Z5748	Chaperonin GroEL	COG0459O	3.5
ybbN	Z0645	Trx-like protein, functions as molecular chaperone	COG3118O	2.4
yrfH	Z4754	Ribosome-associated heat shock protein Hsp15	COG1188J	2.3
lon	Z0545	DNA-binding, ATP-dependent protease La; heat shock K protein	COG0466O	2.2
clpB	Z3886	Protein disaggregation chaperone	COG0542O	3.5
hslU	Z5478	ATP-dependent protease ATP-binding subunit	COG1220O	2.2
hslV	Z5479	ATP-dependent protease peptidase subunit	COG5405O	2.6
Other stress resistance genes
yecG	Z2948	Universal stress protein UspC	COG0589T	2.4
treF	Z4932	Trehalase	COG1626G	2.6
katE	Z2761	Catalase, hydroperoxidase HPII(III)	COG0753P	2.3
yhjA	Z4931	Putative cytochrome c peroxidase	COG1858P	3.2
topA	Z2536	DNA topoisomerase type I, ω protein	COG0550L	2.4
mutS	Z4043	DNA mismatch repair protein	COG0249L	2.1
exo	Z4115	Exonuclease IX	COG0258L	2.6
mutM	Z5059	Formamidopyrimidine-DNA glycosylase	COG0266L	3.6
sms	Z5990	DNA repair protein RadA	COG1066O	2.5
spy	Z2775	Periplasmic protein induced by stress response via Cpx and BaeSR system; similar to CpxP	COG3678UNTP	2.8
yiiO	Z5458	Periplasmic repressor CpxP related to envelope stress	COG3678UNTP	2.1
ykfE	Z0277	Defense, inactivate vertebrate C-type lysozyme	NA	3.2

The COG category was assigned based on the annotation for E. coli O157:H7 strain EDL933. NA, no category assigned.

The ratio of normalized hybridization intensities of the gene in RM6607W to that in RM6607R. All genes listed here passed the significance test as detailed in Materials and Methods.