Table 2.

Oligonucleotide primers used in this study

Primer and use	Sequence (5′→3′)
Overexpression
THI3 Fw HindIII	GGTAAGCTTATGAATTCTAGCTATACACAGAGATATGC
THI3 Rv XhoI	GGCCTCGAGTCAGTATCCAACTTGATTTTTTTTTAGAAGTGGTTGG
PDC5 Fw SpeI	GCCACTAGTATGTCTGAAATAACCTTAGGTAAATATTTATT
PDC5 Rv XhoI	GGCCTCGAG TTATTGTTTAGCGTTAGTAGCGGCAGTCAATT
PDC6 Fw	CACCGCCAACAAAATGTCTGAAATTACTCTTG
PDC6 Rv	TTATTGTTTGGCATTTGTAGCGGCAGTCAATTGC
PDC1 Fw	CACCATGTCTGAAATTACTTTGGGTAAATATTTGTTC
PDC1 Rv	TTATTGCTTAGCGTTGGTAGCAGCAGTC
Gene deletion
PDC1-S1	TTCTACTCATAACCTCACGCAAAATAACACAGTCAAATCACAGCTGAAGCTTCGTACGC
PDC1-S2	AATGCTTATAAAACTTTAACTAATAATTAGAGATTAAATCGCATAGGCCACTAGTGGATCTG
PDC5-S1	ATCAATCTCAAAGAGAACAACACAATACAATAACAAGAAGCAGCTGAAGCTTCGTACGC
PDC5-S2	AAAATACACAAACGTTGAATCATGAGTTTTATGTTAATTAGCATAGGCCACTAGTGGATCTG
PDC6-S1	TAAATAAAAAACCCACGTAATATAGCAAAAACATATTGCCCAGCTGAAGCTTCGTACGC
PDC6-S2	TTTATTTGCAACAATAATTCGTTTGAGTACACTACTAATGGCATAGGCCACTAGTGGATCTG
THI3-S1	TCTAGCTATACACAGAGATATGCACTGCCGAAGTGTATAGCAGCTGAAGCTTCGTACGC
THI3-S2	ATTTACTGCGCTAGAATTTTCGTTCTCCTCTTGCACTTGTGCATAGGCCACTAGTGGATCTG
ARO10-S1	ATGGCACCTGTTACAATTGAAAAGTTCGTAAATCAAGAAGCAGCTGAAGCTTCGTACGC
ARO10-S2	AATATTGCACTTCAGAATGGTGCTCAGTTCTTGGATTGTCGCATAGGCCACTAGTGGATCTG