Table 1.

Primers used for site-directed mutagenesis of scDAOCS

Mutation	Direction	Sequence of mutagenic primera
C155Y	Forward	5′-TCCTCGACTATGAGCCGCTGCTGC-3′
	Reverse	5′-ATAGTCGAGGAATTCCTCGAC-3′
Y184H	Forward	5′-CATCGACAGGTCCATGTGCGGCGCC-3′
	Reverse	5′-ATGGACCTGTCGATGGTCACCCTC-3′
V275I	Forward	5′-ACCTTCTCCATTCCGCTGGCGC-3′
	Reverse	5′-AATGGAGAAGGTGAAGTCCGC-3′
C281Y	Forward	5′-GCGCGCGAGTATGGCTTCGATG-3′
	Reverse	5′-ATACTCGCGCGCCAGCGGGAC-3′
M73T	Forward	5′-GTCCCCACCACCCGCCGCGGCTTC-3′
	Reverse	5′-GGTGGTGGGGACGGGCGAGGTGAC-3′
N304K	Forward	5′-CTACGTGAAAATCCGCCGCACATC-3′
	Reverse	5′-TTTCACGTAGTTGCCCCCGATC-3′
I305M	Forward	5′-CGTGAACATGCGCCGCACATC-3′
	Reverse	5′-CATGTTCACGTAGTTGCCCCCG-3′
I305L	Forward	5′-CGTGAACCTGCGCCGCACATC-3′
	Reverse	5′-CAGGTTCACGTAGTTGCCCCCG-3′
S261A	Forward	5′-GAGGAAGAACACTGCGGAGGTGCGG-3′
	Reverse	5′-GCAGTGTTCTTCCTCCGTCCCAACG-3′
S261M	Forward	5′-GAGGAAGAACACCATGGAGGTGCGG-3′
	Reverse	5′-ATGGTGTTCTTCCTCCGTCCCAACG-3′
Q126M	Forward	5′-CTTCGACCGCATGTACACCGCCTCCCG-3′
	Reverse	5′-CATGCGGTCGAAGTACTGAGTCCAGATCC-3′
T213V	Forward	5′-GGCGCGTTCGTGGACCTGCCCTAC-3′
	Reverse	5′-CACGAACGCGCCGCCGACCTC-3′

^aThe codons corresponding to the mutated residues are underlined.