Frequencies of IFN-γ- and IL-17-producing CD4+ and CD8+ T cells in non-TBi, LTBi, and ATB individuals. CD4+ (A, B, E, and F) and CD8+ (C, D, G, and H) T cells from 24-h whole blood (A to D) and 144-h PBMC (E to H) cultures stimulated with CFP-10 and PPD were intracellularly stained with anti-IFN-γ–PE–Cy7 (A, C, E, and G) and anti-IL-17–PE (B, D, F, and H). The frequencies of IFN-γ- and IL-17-producing CD4+ and CD8+ T cells were evaluated in non-TBi (white circles), LTBi (gray circles), and ATB (black circles). Kruskal-Wallis test with Dunn's multiple comparison test: *, P ≤ 0.05; **, P ≤ 0.01.