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. 2012 Oct;19(10):1667–1676. doi: 10.1128/CVI.00390-12

Fig 3.

Fig 3

Phenotypes of IFN-γ- and IL-17-producing CD4+ T cells in non-TBi, LTBi, and ATB individuals. The phenotypes of IFN-γ- and IL-17-producing CD4+ T cells were evaluated based on the expression of CD45RO and CD27 as described in Materials and Methods. One representative example of the gating strategy used to evaluate the phenotypes of IFN-γ+ and IL-17+ CD4+ T cells from whole-blood 24-h (A) and PBMC 144-h (B) cultures stimulated with CFP-10 from one ATB is shown. The phenotypes of IFN-γ (C, D, G, and H)- and IL-17 (E, F, I, and J)-producing CD4+ T cells from 24-h whole-blood (C to F) and 144-h PBMC (G to J) cultures stimulated with CFP-10 (C, E, G, and I) and PPD (D, F, H, and J) were evaluated by the expression of CD27 and CD45RO. Gray bars, LTBi; black bars, ATB. Two-way ANOVA test with Bonferroni's posttest: *, P ≤ 0.05; **, P ≤ 0.01.