Table 3.
Occurrence of Upc2 amino acid substitutions in predicted translated sequence in fluconazole-resistant clinical ERG11-overexpressing isolates
Upc2 substitution | Isolate | Genotype | Fluconazole MIC (μg/ml) | Expression (fold) of efflux transporter gene (±SE)a |
|||
---|---|---|---|---|---|---|---|
ERG11 | CDR1 | CDR2 | MDR1 | ||||
G648D | 21 | Heterozygous | 32 | 6.2 (±0.2) | 14.7 (±5.5) | 883.3 (±301.9) | 10.4 (±3.1) |
38 | Heterozygous | 64 | 3.8 (±0.3) | 3.9 (±0.7) | 111.0 (±57.1) | 1.4 (±0.2) | |
47 | Heterozygous | 128 | 7.9 (±1.2) | 11.8 (±5.1) | 327.0 (±166.3) | 8.2 (±3.1) | |
52 | Heterozygous | 256 | 3.7 (±1.4) | 1.2 (±0.3) | 17.1 (±3.2) | 0.3 (±0.1) | |
59 | Heterozygous | >256 | 7.2 (±1.0) | 6.5 (±0.3) | 48.5 (±0.3) | 8.0 (±0.2) | |
71 | Heterozygous | >256 | 9.4 (±1.1) | 5.6 (±1.9) | 149.8 (±56.5) | 4.6 (±1.8) | |
G648S | 25 | Heterozygous | 64 | 3.6 (±0.6) | 1.1 (±0.5) | 2.8 (±1.1) | 160.5 (±78.9) |
35 | Heterozygous | >256 | 4.4 (±1.3) | 1.2 (±0.4) | 0.7 (±0.3) | 79.9 (±22.8) | |
55 | Homozygous | >256 | 2.6 (±0.6) | 1.7 (±1.5) | 26.2 (±17.4) | 0.9 (±3.2) | |
56 | Homozygous | >256 | 2.1 (±1.0) | 8.0 (±3.1) | 167.8 (±94.0) | 2.5 (±0.9) | |
57 | Homozygous | >256 | 19.4 (±12.4) | 10.2 (±3.5) | 263.3 (±106.0) | 12.9 (±5.2) | |
58 | Homozygous | >256 | 6.6 (±1.5) | 9.7 (±4.2) | 226.6 (±131.7) | 3.8 (±1.2) | |
65 | Heterozygous | >256 | 4.2 (±0.3) | 9.8 (±1.5) | 294.9 (±128.4) | 2.4 (±0.1) | |
67 | Heterozygous | >256 | 4.7 (±0.7) | 7.4 (±2.8) | 105.1 (±43.3) | 1.2 (±0.5) | |
69 | Heterozygous | >256 | 5.6 (±0.9) | 7.2 (±0.5) | 136.8 (±69.3) | 1.4 (±0.5) | |
A643T | 13 | Homozygous | 16 | 2.4 (±1.1) | 6.4 (±2.9) | 185.9 (±80.9) | 2.4 (1.6) |
A643V | 30 | Heterozygous | 64 | 4.6 (±1.6) | 1.4 (±0.3) | 18.0 (±15.7) | 18.9 (±16.7) |
68 | Heterozygous | >256 | 4.9 (±0.4) | 6.3 (±2.2) | 64.5 (±31.8) | 216.3 (±98.9) | |
A646V | 45 | Heterozygous | 128 | 3.5 (±0.3) | 4.8 (±0.3) | 147.8 (±71.7) | 15.5(±7.7) |
67 | Heterozygous | >256 | 4.7 (±0.7) | 7.4 (±2.8) | 105.1 (±43.3) | 1.2 (±0.5) | |
Y642F | 33 | Homozygous | 36 | 12.6 (±1.8) | 6.8 (±2.0) | 201.5 (±129.0) | 1.5 (±0.5) |
34 | Homozygous | 37 | 7.5 (±0.6) | 5.4 (2.5) | 126.8 (±62.6) | 1.2 (±0.5) | |
W478C | 15 | Heterozygous | 16 | 8.1 (±2.7) | 15.9 (±3.8) | 1415.1 (±400.1) | 4.0 (±3.0) |
27 | Heterozygous | 64 | 6.2 (±2.8) | 14.2 (±4.7) | 629.7 (±255.3) | 1.1 (±0.1) | |
28 | Heterozygous | 64 | 18.1 (±7.3) | 18.1 (±6.3) | 1439.7 (±570.8) | 23.2 (±20.6) | |
G304R | 18 | Heterozygous | 32 | 5.6 (±1.2) | 10.4 (±2.4) | 575.0 (±117.2) | 1.0 (±0.3) |
19 | Heterozygous | 32 | 5.2 (±1.4) | 10.0 (±4.8) | 401.3 (±204.5) | 4.6 (±3.0) |
qRT-PCR was performed in triplicate.