Table 4.
MICs of a panel of antifungals in YPD against strains expressing UPC2 mutant alleles
Strain | Relevant genotype | MIC (μg/ml) after indicated incubation (h)a |
|||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
FCZ |
ICZ |
VCZ |
TBF |
AMB |
|||||||
24 | 48 | 24 | 48 | 24 | 48 | 24 | 48 | 24 | 48 | ||
SC5314 | UPC2-1/UPC2-2 | 0.5 | 4 | 0.016 | 0.023 | 0.008 | 0.032 | 0.5 | 2 | 0.0313 | 0.125 |
UPC2M4A | upc2-1Δ::FRT/upc2-2Δ::FRT | 0.023 | 0.032 | 0.002 | 0.004 | 0.002 | 0.002 | <0.016 | <0.016 | 0.0313 | 0.5 |
11A8A2A | UPC2G648S::FRT/UPC2G648S::FRT | 4 | >256 | 0.5 | 0.75 | 0.25 | >32 | 2 | 4 | 0.016 | 0.125 |
SC11A1A | UPC2/UPC2G648S::FRT | 1 | >256 | 0.032 | 0.19 | 0.047 | >32 | 2 | 4 | 0.0625 | 0.25 |
28A7A10A | UPC2A643V::FRT/UPC2A643V::FRT | 2 | >256 | 0.125 | >32 | 0.25 | >32 | 1 | 4 | 0.016 | 0.125 |
SC28A3A | UPC2/UPC2A643V::FRT | 1 | 2 | 0.023 | 0.047 | 0.047 | >32 | 1 | 2 | 0.0313 | 0.25 |
YFA3A2K1 | UPC2Y642F::FRT/UPC2Y642F::FRT | 2 | >256 | 0.047 | 0.5 | 0.064 | >32 | 1 | 4 | 0.0625 | 0.5 |
SCYFA2A | UPC2/UPC2Y642F::FRT | 0.75 | 1 | 0.023 | 0.032 | 0.023 | 0.19 | 1 | 2 | 0.0313 | 0.25 |
AVA1A16A | UPC2A646V::FRT/UPC2A646V::FRT | 1.5 | >256 | 0.064 | 0.25 | 0.064 | >32 | 1 | 4 | 0.0313 | 0.25 |
SCAVA4 | UPC2/UPC2A646V::SAT1-FLIP | 0.5 | 1.5 | 0.016 | 0.032 | 0.016 | 0.064 | 1 | 2 | 0.0313 | 0.5 |
22A1A13A | UPC2G304R::FRT/UPC2G304R::FRT | 0.19 | 0.75 | 0.012 | 0.023 | 0.008 | 0.032 | 1 | 2 | 0.0313 | 0.5 |
SC22A3A | UPC2/UPC2G304R::FRT | 0.75 | >256 | 0.012 | 0.094 | 0.023 | >32 | 1 | 4 | 0.0313 | 0.25 |
25B2D1 | UPC2W478C::FRT/UPC2W478C::FRT | 1 | 2 | 0.047 | 0.047 | 0.016 | 0.5 | 0.25 | 1 | 0.0313 | 0.25 |
SC25A1 | UPC2/UPC2W478C::FRT | 0.5 | 1 | 0.016 | 0.016 | 0.008 | 0.032 | 0.5 | 1 | 0.016 | 0.125 |
SCUPC2R14A | UPC2G648D::FRT/UPC2G648D::FRT | 8 | >256 | >32 | >32 | 0.064 | >32 | 2 | 4 | 0.016 | 0.125 |
SCUPC2R12A | UPC2G648D::FRT/UPC2-2 | 1.5 | >256 | 0.25 | >32 | 0.047 | >32 | 1 | 2 | 0.0313 | 0.25 |
SCUPC2R34A | UPC2A643T::FRT/UPC2A643T::FRT | 1.5 | >256 | 2 | >32 | 0.064 | >32 | 2 | 2 | 0.016 | 0.125 |
SCUPC2R32A | UPC2A643T::FRT/UPC2-2 | 2 | >256 | 0.016 | 0.032 | 0.012 | >32 | 1 | 2 | 0.016 | 0.125 |
Susceptibility was tested at 24 and at 48 h. Azole MICs were determined by Etest method, while terbinafine and amphotericin B MICs were determined by broth microdilution methods. FCZ, fluconazole; ICZ, itraconazole; VCZ, voriconazole; TBF, terbinafine; AMB, amphotericin B.