Fig 4.

Neurospora Gα proteins physically interact with Gβγ in vivo. Wild-type (74a) and GNG-1-FLAG (5A) strains were grown for 16 h in submerged VM cultures. The particulate fraction was isolated and solubilized using lauryl maltoside and immunoprecipitated using anti-FLAG antibody coupled to agarose beads. Samples of the solubilized particulate fraction (Input), flowthrough (Unbound), and bound (Bound) fractions were subjected to Western analysis using GNA-1, GNA-2, and GNA-3 antibodies as described in Materials and Methods.