Fig 1.

(A) qRT-PCR analysis of gliK expression in A. fumigatus ATCC 46645 and the ΔgliK⁴⁶⁶⁴⁵ mutant in AMM at 24 h at 37°C. (B) Quantitative RT-PCR (qRT-PCR) analysis of gliK expression at 24, 48, and 72 h in A. fumigatus ATCC 46645 cultured in various media at 37°C. (C) qRT-PCR analysis of gliK expression in A. fumigatus ATCC 46645 and the ΔgliK⁴⁶⁶⁴⁵ mutant, respectively, in AMM at selected time points at 37°C. (D) qRT-PCR analysis of gliK expression in A. fumigatus ATCC 46645 in AMM at 24 h at 37°C, with and without exogenous gliotoxin spiked into the media. Column 1, gliK expression in A. fumigatus ATCC 46645; column 2, gliK expression in A. fumigatus ATCC 46645 spiked with gliotoxin (5 μg/ml final concentration). (E) qRT-PCR analysis of gliK expression in A. fumigatus ATCC 46645 in AMM at 24 h at 37°C, with and without hydrogen peroxide spiked into the media. Column 1, gliK expression in A. fumigatus ATCC 46645; column 2, gliK expression in A. fumigatus ATCC 46645 spiked with 1 mM hydrogen peroxide (final concentration). SAB, Sabouraud medium.