Fig 4.
RP-HPLC confirms gliotoxin absence from A. fumigatus ΔgliK. Standard gliotoxin with a retention time of 13.51 min (A), ATCC 26933 with gliotoxin at an Rt of 13.50 min (B), and the ΔgliK26933 mutant lacking a corresponding gliotoxin peak (C). Detection was at 254 nm. (D) MALDI-TOF analysis reveals diacetamidofluorescein-gliotoxin [GT-(AF)2] (m/z 1102.94) (12) following reduction and alkylation of organic extracts from A. fumigatus ATCC 26933. (E) GT-(AF)2 is not present in organic extracts of the A. fumigatus ΔgliK26933 mutant.