Table 4.
Recovery of and detection limits for Giardia and Cryptosporidium spp. in dairy wastewaters as determined by qPCR with low and high total solids
| Target organism | Total solids (g liter−1) | Wastewater titer range after spiking (log10 cysts/oocysts ml−1 wastewater) | Range of recovery values (%) | Mean recovery (%)a | qPCR detection limit (log10 cysts/oocysts ml−1) |
|---|---|---|---|---|---|
| Giardia spp. | 3.6 | 3–6 | 8–20 | 15 | 130 |
| 12.7 | 15–25 | 19 | 182 | ||
| Cryptosporidium spp. | 3.6 | 3–6 | 22–110 | 61 | 1,485 |
| 12.7 | 71–426 | 210 | 1,689 |
a
Data represent mean values as determined by spiking triplicate wastewater samples with pathogens at several titer levels.