Table 1.
Differentially regulated Salmonella Typhimurium SL1344 genes after 22 h of dehydrationa
| Category, gene, and/or locus tagb | Gene/protein annotation | Fold changec |
|---|---|---|
| Translation/ribosomal structure | ||
| deaD (STM3280.S) | Cysteine sulfinate desulfinase | 2.68 |
| ftsJ (STM3297) | 23S rRNA methyltransferase | 2.89 |
| rpsD (STM3416) | 30S ribosomal protein S4 | 2.40 |
| rpsK (STM3417) | 30S ribosomal protein S11 | 2.29 |
| rplO (STM3421) | 50S ribosomal protein L15 | 2.14 |
| rpsH (STM3426) | 30S ribosomal protein S8 | 2.39 |
| rplE (STM3428) | 50S ribosomal protein L5 | 3.16 |
| rplX (STM3429) | 50S ribosomal protein L24 | 2.69 |
| rpsQ (STM3431) | 30S ribosomal protein S17 | 2.25 |
| rplP (STM3433) | 50S ribosomal protein L16 | 2.92 |
| rpsC (STM3434) | 30S ribosomal protein S3 | 2.75 |
| rplB (STM3437) | 50S ribosomal protein L2 | 3.49 |
| rplW (STM3438) | 50S ribosomal protein L23 | 2.62 |
| rplD (STM3439) | 50S ribosomal protein L4 | 2.82 |
| rplC (STM3440) | 50S ribosomal protein L3 | 2.84 |
| rpsJ (STM3441) | 30S ribosomal protein S10 | 2.69 |
| fusA (STM3446) | Elongation factor EF-2 | 1.93 |
| rpsG (STM3447) | 30S ribosomal protein S7 | 2.16 |
| rplK (STM4149) | 50S ribosomal protein L11 | 2.33 |
| rplA (STM4150) | 50S ribosomal protein L1 | 2.02 |
| rplJ (STM4151) | 50S ribosomal protein L10 | 2.02 |
| miaA (STM4360) | tRNA delta(2)-isopentenylpyrophosphate transferase | 1.97 |
| Amino acid transport and metabolism | ||
| hisD (STM2072) | Histidinol dehydrogenase | 2.44 |
| hisC (STM2073) | Histidinol-phosphate aminotransferase | 2.67 |
| hisB (STM2074) | Imidazole glycerol-phosphate dehydratase/histidinol phosphatase | 2.23 |
| hisH (STM2075) | Imidazole glycerol phosphate synthase subunit HisH | 2.34 |
| hisA (STM2076) | 1-(5-Phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase | 2.39 |
| hisF (STM2077) | Imidazole glycerol phosphate synthase subunit | 2.68 |
| glpB (STM2285) | Anaerobic glycerol-3-phosphate dehydrogenase subunit B | 2.18 |
| nifS (STM2543) | Cysteine desulfurase | 2.23 |
| argA (STM2992) | N-Acetylglutamate synthase | 2.30 |
| ygjU (STM3225) | Putative dicarboxylate permease | 2.04 |
| argG (STM3290.S) | Argininosuccinate synthase | 2.52 |
| gltB (STM3330) | Glutamate synthase large subunit | 2.42 |
| gltD (STM3331) | Glutamate synthase small subunit | 2.68 |
| prlC (STM3594) | Oligopeptidase A | 2.18 |
| argE (STM4120) | Acetylornithine deacetylase | 1.99 |
| argC (STM4121) | N-Acetyl-gamma-glutamyl-phosphate reductase | 2.26 |
| argB (STM4122) | Acetylglutamate kinase | 3.03 |
| argD (STM1303) | Bifunctional aconitate hydratase 2,2-methylisocitrate dehydratase | 1.83 |
| argI (STM4469) | Ornithine carbamoyltransferase | 1.83 |
| Stress response | ||
| dnaK (STM0012) | Molecular chaperone DnaK | 3.05 |
| hflB (STM3296) | ATP-dependent zinc-metallo protease | 2.84 |
| yhgI (STM3511) | Putative thioredoxin-like protein | 2.34 |
| ydaA (STM 1661) | Universal stress protein UspE | 1.90 |
| ibpA (STM3809.S) | Small heat shock protein | 2.75 |
| hslU (STM4091) | ATP-dependent protease heat shock protein | 2.19 |
| groEL (STM4330) | Chaperonin GroEL | 2.11 |
| sufD (STM1372) | Cysteine desulfurase modulator | 1.86 |
| Transcription | ||
| rpoE (STM2640) | RNA polymerase sigma factor | 2.53 |
| rpoA (STM3415) | DNA-directed RNA polymerase alpha subunit | 2.16 |
| rpoH (STM3568) | RNA polymerase sigma factor | 2.48 |
| rpoB (STM4153) | DNA-directed RNA polymerase beta subunit | 2.37 |
| rpoC (STM4154) | DNA-directed RNA polymerase beta subunit | 2.36 |
| rseA (STM2639) | Sigma factor RpoE negative regulatory protein RseA | 2.05 |
| fnr (STM1660.S) | Transcriptional regulator | 1.84 |
| greA (STM3299) | Transcription elongation factor | 1.82 |
| Energy production and conversion | ||
| icdA (STM1238) | Isocitrate dehydrogenase | 2.35 |
| fumA (STM1468) | Fumarase A | 2.17 |
| glpC (STM2286) | sn-Glycerol-3-phosphate dehydrogenase K small subunit | 2.43 |
| nifU (STM2542) | NifU-like protein | 2.70 |
| glpK (STM4086) | Glycerol kinase | 3.19 |
| aceB (STM4183) | Malate synthase | 2.92 |
| aceA (STM4184) | Isocitrate lyase | 3.34 |
| Inorganic ion transport | ||
| kdpC (STM0704) | Potassium-transporting ATPase C chain | 4.51 |
| kdpB (STM0705) | Potassium-transporting ATPase subunit B | 5.02 |
| kdpA (STM0706) | Potassium-transporting ATPase subunit A | 4.67 |
| cutC (STM1907) | Copper homeostasis protein | 0.24 |
| Carbohydrate transport and metabolism | ||
| glgA (STM3535) | Glycogen synthase | 1.89 |
| mglB (STM2190) | Galactose transport protein | 2.50 |
| pps (STM1349) | Phosphoenolpyruvate synthase | 2.00 |
| mtlA (STM3685) | Mannitol-specific enzyme IIABC component | 2.19 |
| Lipid transport and metabolism | ||
| ddg (STM2401) | Lipid A biosynthesis lauroyl acyltransferase | 3.69 |
| nlpD (STM2925) | Lipoprotein | 2.20 |
| nlpI (STM3281) | Lipoprotein | 1.97 |
| Other or unknown function | ||
| prpE (STM0371) | Putative acetyl coenzyme A synthetase | 1.93 |
| prpD (STM0370) | 2-Methylcitrate dehydratase | 1.83 |
| phoL (STM0669) | Putative phosphate starvation-inducible protein | 1.90 |
| yceD (STM1190) | Putative metal-binding protein | 2.08 |
| ycjX (STM1685) | Putative ATPase | 1.94 |
| sixA (STM2387) | Phosphohistidine phosphatase | 1.96 |
| iscA (STM2541) | Iron-sulfur cluster assembly protein | 3.13 |
| yggN (STM3107) | Putative periplasmic protein | 3.06 |
| exbB (STM3159) | Energy transduction protein | 2.05 |
| pckA (STM3500) | Phosphoenolpyruvate carboxykinase | 1.90 |
| slsA (STM3761) | Putative inner membrane protein | 3.35 |
| aceK (STM4185) | Bifunctional isocitrate dehydrogenase kinase/phosphatase protein | 2.69 |
| phoH (STM1126) | Phosphate starvation-inducible protein | 1.95 |
| minE (STM1816) | Cell division topological specificity factor MinE | 1.85 |
| yahO (STM0366) | Periplasmic protein of unknown function | 1.80 |
| ybeL (STM0653) | Hypothetical protein | 1.85 |
| tke1 (STM_sRNA) | Small RNA | 0.53 |
| STM1731 | Putative catalase | 0.54 |
| Virulence | ||
| parA (PSLT052) | Plasmid partition protein A | 0.43 |
| traN (PSLT095) | Mating pair stabilization protein | 0.44 |
| trbH (PSLT105) | Conjugative transfer protein | 0.41 |
| PSLT068 | Putative ParB-like nuclease | 0.51 |
a
All of the listed genes were significantly differentiated in expression compared to the control at a P value of <0.05.
b
Derived from S. Typhimurium strain LT2 (NCBI site).
c
The fold change is the ratio of the gene expression level of the dehydrated cells to that of the control maintained in water. Values lower than 1 represent decreased expression.