Fig 1.

Biofilm formation measured by XTT reduction assays. C. neoformans biofilms were grown in biofilm-inducing medium containing one of the following cation chelators at concentrations of 0 to 25 mM (with major target cations in parentheses): EDTA (Ca²⁺ and Mg²⁺), EGTA (Ca²⁺ and Mg²⁺), DFO (Fe²⁺), or TETA (Cu²⁺). Cells were incubated with the chelator for 24 h, and XTT assays were subsequently performed.